FANCG monoclonal antibody (M01), clone 2C8
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More Files
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Western Blot (Cell lysate)
FANCG monoclonal antibody (M01), clone 2C8 Western Blot analysis of FANCG expression in Hela S3 NE ( Cat # L013V3 ).
Western Blot (Transfected lysate)
Western Blot analysis of FANCG expression in transfected 293T cell line by FANCG monoclonal antibody (M01), clone 2C8.
Lane 1: FANCG transfected lysate(68.6 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of FANCG transfected lysate using anti-FANCG monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FANCG MaxPab rabbit polyclonal antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of FANCG over-expressed 293 cell line, cotransfected with FANCG Validated Chimera RNAi ( Cat # H00002189-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FANCG monoclonal antibody (M01), clone 2C8 (Cat # H00002189-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to FANCG on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (94.16 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant FANCG.
Immunogen
FANCG (AAH11623, 1 a.a. ~ 622 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSRQTTSVGSSCLDLWREKNDRLVRQAKVAQNSGLTLRRQQLAQDALEGLRGLLHSLQGLPAAVPVLPLELTVTCNFIILRASLAQGFTEDQAQDIQRSLERVLETQEQQGPRLEQGLRELWDSVLRASCLLPELLSALHRLVGLQAALWLSADRLGDLALLLETLNGSQSGASKDLLLLLKTWSPPAEELDAPLTLQDAQGLKDVLLTAFAYRQGLQELITGNPDKALSSLHEAASGLCPRPVLVQVYTALGSCHRKMGNPQRALLYLVAALKEGSAWGPPLLEASRLYQQLGDTTAELESLELLVEALNVPCSSKAPQFLIEVELLLPPPDLASPLHCGTQSQTKHILASRCLQTGRAGDAAEHYLDLLALLLDSSEPRFSPPPSPPGPCMPEVFLEAAVALIQAGRAQDALTLCEELLSRTSSLLPKMSRLWEDARKGTKELPYCPLWVSATHLLQGQAWVQLGAQKVAISEFSRCLELLFRATPEEKEQGAAFNCEQGCKSDAALQQLRAAALISRGLEWVASGQDTKALQDFLLSVQMCPGNRDTYFHLLQTLKRLDRRDEATALWWRLEAQTKGSHEDALWSLPLYLESYLSWIRPSDRDAFLEEFRTSLPKSCDL
Host
Mouse
Reactivity
Human
Isotype
IgG2a kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (94.16 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
FANCG monoclonal antibody (M01), clone 2C8 Western Blot analysis of FANCG expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Transfected lysate)
Western Blot analysis of FANCG expression in transfected 293T cell line by FANCG monoclonal antibody (M01), clone 2C8.
Lane 1: FANCG transfected lysate(68.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of FANCG transfected lysate using anti-FANCG monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FANCG MaxPab rabbit polyclonal antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of FANCG over-expressed 293 cell line, cotransfected with FANCG Validated Chimera RNAi ( Cat # H00002189-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FANCG monoclonal antibody (M01), clone 2C8 (Cat # H00002189-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to FANCG on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — FANCG
Entrez GeneID
2189GeneBank Accession#
BC011623Protein Accession#
AAH11623Gene Name
FANCG
Gene Alias
FAG, XRCC9
Gene Description
Fanconi anemia, complementation group G
Omim ID
602956Gene Ontology
HyperlinkGene Summary
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G. [provided by RefSeq
Other Designations
DNA repair protein XRCC9|OTTHUMP00000021319|X-ray repair complementing defective repair in Chinese hamster cells 9|X-ray repair, complementing defective, in Chinese hamster, 9
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Interactome
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Disease
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Publication Reference
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Areca Nut Induces miR-23a and Inhibits Repair of DNA Double-Strand Breaks by Targeting FANCG.
Tsai YS, Lin CS, Chiang SL, Lee CH, Lee KW, Ko YC.
Toxicological Sciences 2011 Oct; 123(2):480.
Application：WB-Tr, Human, 293 cells.
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Stimulation of Homology-directed Repair at I-SceI-induced DNA breaks during the Permissive Life Cycle of Human Cytomegalovirus.
Kulkarni AS, Fortunato EA.
J Virol 2011 Apr; 85:6049.
Application：IF, WB-Tr, Human, HFFs.
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Four human FANCG polymorphic variants show normal biological function in hamster CHO cells.
Hinz JM, Nham PB, Yamada NA, Tebbs RS, Salazar EP, Hinz AK, Mohrenweiser HW, Jones IM, Thompson LH.
Mutation Research 2006 Sep; 602(1-2):34.
Application：WB, Human, AA8, KO40, MMC-R3 cells.
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Areca Nut Induces miR-23a and Inhibits Repair of DNA Double-Strand Breaks by Targeting FANCG.
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