ZFYVE20 polyclonal antibody
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of ZFYVE20.
Immunogen
A synthetic peptide corresponding to human ZFYVE20.
Sequence
C-ELKHTLAKQKGGTD
Host
Goat
Theoretical MW (kDa)
88.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ZFYVE20 polyclonal antibody (Cat # PAB6458) staining (0.3 ug/mL) of A-431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ZFYVE20
Entrez GeneID
64145Protein Accession#
NP_071735Gene Name
ZFYVE20
Gene Alias
FLJ34993, MGC126210, Rabenosyn-5
Gene Description
zinc finger, FYVE domain containing 20
Omim ID
609511Gene Ontology
HyperlinkGene Summary
O
Other Designations
FYVE-finger-containing Rab5 effector protein rabenosyn-5|OTTHUMP00000160299
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Interactomes
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Pathways
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Publication Reference
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Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain.
Nielsen E, Christoforidis S, Uttenweiler-Joseph S, Miaczynska M, Dewitte F, Wilm M, Hoflack B, Zerial M.
The Journal of Cell Biology 2000 Oct; 151(3):601.
Application:WB-Ce, Human, A431 cells.
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Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain.
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