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Last updated: 2017/6/25
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TRIM5 polyclonal antibody

  • Catalog # : PAB6383
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of TRIM5.
  • Immunogen:
  • A synthetic peptide corresponding to human TRIM5.
  • Sequence:
  • ASGILVNVKEEVTC
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 56.3, 40.1, 37.7
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize all three human isoforms of this protein.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:16000)
    Western Blot (1-3 ug/mL)
    Immunohistochemistry (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • TRIM5 polyclonal antibody (Cat # PAB6383) staining (1 ug/mL) of human brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • TRIM5 polyclonal antibody (Cat # PAB6383) staining (1 ug/mL) of paraffin embedded human tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.A) positive cells next to a secondary follicle, B) positive cells below tonsillar epithelium.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_149023.1;NP_149083.1;NP_149084.1
  • Gene Name:
  • TRIM5
  • Gene Alias:
  • RNF88,TRIM5alpha
  • Gene Description:
  • tripartite motif-containing 5
  • Gene Summary:
  • The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Five alternatively spliced transcript variants for this gene have been described. However, the full length nature of two of the variants has not been determined. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000069811,OTTHUMP00000069812,tripartite motif protein TRIM,tripartite motif protein TRIM5,tripartite motif-containing protein 5 alpha
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