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Last updated: 2017/8/13
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STRADB polyclonal antibody

  • Catalog # : PAB6344
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of STRADB.
  • Immunogen:
  • A synthetic peptide corresponding to human STRADB.
  • Sequence:
  • CDFPDEKDSYWEF
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 47, 31.2
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize expected to recognize both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP-beta (AAF71042.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:32000)
    Western blot (1-3 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • STRADB polyclonal antibody (Cat # PAB6344) staining (1ug/ml) of human heart lysate (RIPA buffer, 30ug total protein per lane) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • HEK293 overexpressing STRADB and probed with STRADB polyclonal antibody (Cat # PAB6344) (mock transfection in first lane), tested by Origene.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • STRADB polyclonal antibody (Cat # PAB6344) (3.8 ug/mL) staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_061041.2;AAF71042.1
  • Gene Name:
  • STRADB
  • Gene Alias:
  • ALS2CR2,CALS-21,ILPIP,ILPIPA,MGC102916,PAPK,PRO1038
  • Gene Description:
  • STE20-related kinase adaptor beta
  • Gene Summary:
  • This gene encodes a protein that belongs to the serine/threonine protein kinase STE20 subfamily. One of the active site residues in the protein kinase domain of this protein is altered, and it is thus a pseudokinase. This protein is a component of a complex involved in the activation of serine/threonine kinase 11, a master kinase that regulates cell polarity and energy-generating metabolism. This complex regulates the relocation of this kinase from the nucleus to the cytoplasm, and it is essential for G1 cell cycle arrest mediated by this kinase. The protein encoded by this gene can also interact with the X chromosome-linked inhibitor of apoptosis protein, and this interaction enhances the anti-apoptotic activity of this protein via the JNK1 signal transduction pathway. Two pseudogenes, located on chromosomes 1 and 7, have been found for this gene. [provided by RefSeq
  • Other Designations:
  • ILP-interacting protein ILPIPA,STRAD beta,amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2,pseudokinase ALS2CR2
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