STRADB polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
STRADB polyclonal antibody (Cat # PAB6344) staining (1ug/ml) of human heart lysate (RIPA buffer, 30ug total protein per lane) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing STRADB and probed with STRADB polyclonal antibody (Cat # PAB6344) (mock transfection in first lane), tested by Origene.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
STRADB polyclonal antibody (Cat # PAB6344) (3.8 ug/mL) staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of STRADB.
Immunogen
A synthetic peptide corresponding to human STRADB.
Sequence
CDFPDEKDSYWEF
Host
Goat
Theoretical MW (kDa)
47, 31.2
Reactivity
Human
Specificity
This antibody is expected to recognize expected to recognize both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP-beta (AAF71042.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
STRADB polyclonal antibody (Cat # PAB6344) staining (1ug/ml) of human heart lysate (RIPA buffer, 30ug total protein per lane) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing STRADB and probed with STRADB polyclonal antibody (Cat # PAB6344) (mock transfection in first lane), tested by Origene.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
STRADB polyclonal antibody (Cat # PAB6344) (3.8 ug/mL) staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — STRADB
Entrez GeneID
55437Protein Accession#
NP_061041.2;AAF71042.1Gene Name
STRADB
Gene Alias
ALS2CR2, CALS-21, ILPIP, ILPIPA, MGC102916, PAPK, PRO1038
Gene Description
STE20-related kinase adaptor beta
Omim ID
607333Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that belongs to the serine/threonine protein kinase STE20 subfamily. One of the active site residues in the protein kinase domain of this protein is altered, and it is thus a pseudokinase. This protein is a component of a complex involved in the activation of serine/threonine kinase 11, a master kinase that regulates cell polarity and energy-generating metabolism. This complex regulates the relocation of this kinase from the nucleus to the cytoplasm, and it is essential for G1 cell cycle arrest mediated by this kinase. The protein encoded by this gene can also interact with the X chromosome-linked inhibitor of apoptosis protein, and this interaction enhances the anti-apoptotic activity of this protein via the JNK1 signal transduction pathway. Two pseudogenes, located on chromosomes 1 and 7, have been found for this gene. [provided by RefSeq
Other Designations
ILP-interacting protein ILPIPA|STRAD beta|amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2|pseudokinase ALS2CR2
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Interactome
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Publication Reference
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ILPIP, a novel anti-apoptotic protein that enhances XIAP-mediated activation of JNK1 and protection against apoptosis.
Sanna MG, da Silva Correia J, Luo Y, Chuang B, Paulson LM, Nguyen B, Deveraux QL, Ulevitch RJ.
The Journal of Biological Chemistry 2002 Jun; 277(34):30454.
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ILPIP, a novel anti-apoptotic protein that enhances XIAP-mediated activation of JNK1 and protection against apoptosis.
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