SART1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
SART1 polyclonal antibody (Cat # PAB6298) staining (0.03 ug/mL) of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SART1.
Immunogen
A synthetic peptide corresponding to human SART1.
Sequence
GSSKKHRGEKEAA-C
Host
Goat
Theoretical MW (kDa)
90.3
Reactivity
Human
Specificity
This antibody is expected to recognize both the human SART1(259) and SART1(800) proteins.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SART1 polyclonal antibody (Cat # PAB6298) staining (0.03 ug/mL) of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SART1
Entrez GeneID
9092Protein Accession#
NP_005137.1Gene Name
SART1
Gene Alias
Ara1, HOMS1, MGC2038, SART1259, SNRNP110, Snu66
Gene Description
squamous cell carcinoma antigen recognized by T cells
Omim ID
605941Gene Ontology
HyperlinkGene Summary
This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway. [provided by RefSeq
Other Designations
IgE autoantigen|SART1(259) protein|SART1(800) protein|U4/U6.U5 tri-snRNP-associated 110 kDa protein|small nuclear ribonucleoprotein 110kDa (U4/U6.U5)|squamous cell carcinoma antigen recognised by T cells|squamous cell carcinoma antigen recognized by T cel
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Interactome
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Disease
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Publication Reference
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A proteomic study of SUMO-2 target proteins.
Vertegaal AC, Ogg SC, Jaffray E, Rodriguez MS, Hay RT, Andersen JS, Mann M, Lamond AI.
The Journal of Biological Chemistry 2004 Aug; 279(32):33791.
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A proteomic study of SUMO-2 target proteins.
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