CBR1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
CBR1 polyclonal antibody (Cat # PAB6202) staining (0.2 ug/mL) of human liver lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CBR1 polyclonal antibody (Cat # PAB6202) staining (3 ug/mL) of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CBR1.
Immunogen
A synthetic peptide corresponding to C-terminus of human CBR1.
Sequence
C-HGQFVSEKRVEQW
Host
Goat
Theoretical MW (kDa)
30.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.2-0.5 ug/mL)
Immunohistochemistry (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CBR1 polyclonal antibody (Cat # PAB6202) staining (0.2 ug/mL) of human liver lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CBR1 polyclonal antibody (Cat # PAB6202) staining (3 ug/mL) of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CBR1
Entrez GeneID
873Protein Accession#
NP_001748.1Gene Name
CBR1
Gene Alias
CBR, SDR21C1, hCBR1
Gene Description
carbonyl reductase 1
Omim ID
114830Gene Ontology
HyperlinkGene Summary
Carbonyl reductase is one of several monomeric, NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds. This enzyme is widely distributed in human tissues. Another carbonyl reductase gene, CRB3, lies close to this gene on chromosome 21q. [provided by RefSeq
Other Designations
carbonyl reductase (NADPH) 1|prostaglandin 9-ketoreductase|prostaglandin-E(2) 9-reductase|short chain dehydrogenase/reductase family 21C, member 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Increased brain protein levels of carbonyl reductase and alcohol dehydrogenase in Down syndrome and Alzheimer's disease.
Balcz B, Kirchner L, Cairns N, Fountoulakis M, Lubec G.
Journal of Neural Transmission. Supplementum 2001 Feb; 61:193.
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Increased brain protein levels of carbonyl reductase and alcohol dehydrogenase in Down syndrome and Alzheimer's disease.
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