GRB7 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GRB7.
Immunogen
A synthetic peptide corresponding to human GRB7.
Sequence
ELDLSPPHLSSSPE-C
Host
Goat
Theoretical MW (kDa)
59.7
Reactivity
Human
Specificity
This antibody recognizes the longer isoform of this protein in A431 lysate.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
GRB7 polyclonal antibody (Cat # PAB6157) staining (2 ug/mL) of A-431 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing GRB7 and probed with GRB7 polyclonal antibody (Cat # PAB6157) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GRB7
Entrez GeneID
2886Protein Accession#
NP_005301Gene Name
GRB7
Gene Alias
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Gene Description
growth factor receptor-bound protein 7
Omim ID
601522Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Alternative splicing results in multiple transcript variants encoding different isoforms, although the full-length natures of only two of the variants have been determined to date. [provided by RefSeq
Other Designations
OTTHUMP00000164352
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Interactome
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Disease
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Publication Reference
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High-efficiency expression/cloning of epidermal growth factor-receptor-binding proteins with Src homology 2 domains.
Margolis B, Silvennoinen O, Comoglio F, Roonprapunt C, Skolnik E, Ullrich A, Schlessinger J.
PNAS 1992 Oct; 89(19):8894.
Application:WB-Ti, Mouse, Kidney, Livers, Mouse tissues.
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High-efficiency expression/cloning of epidermal growth factor-receptor-binding proteins with Src homology 2 domains.
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