NCF1 polyclonal antibody
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of NCF1.
Immunogen
A synthetic peptide corresponding to human NCF1.
Sequence
C-SESTKRKLASAV
Host
Goat
Theoretical MW (kDa)
44.7
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
NCF1 polyclonal antibody (Cat # PAB6021) staining (0.2 ug/mL) of U-937 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NCF1
Entrez GeneID
653361Protein Accession#
NP_000256.3Gene Name
NCF1
Gene Alias
FLJ79451, NCF1A, NOXO2, SH3PXD1A, p47phox
Gene Description
neutrophil cytosolic factor 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq
Other Designations
NADPH oxidase organizer 2
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Interactomes
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Pathways
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Diseases
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Publication Reference
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p47phox-Dependent Reactive Oxygen Species Stimulate Nuclear Translocation of the FoxO1 Transcription Factor During Metabolic Inhibition in Cardiomyoblasts.
Ter Horst EN, Hahn NE, Geerts D, Musters RJP, Paulus WJ, van Rossum AC, Meischl C, Piek JJ, Niessen HWM, Krijnen PAJ.
Cell Biochemistry and Biophysics 2018 Sep; 76(3):401.
Application:WB-Tr, Rat, H9c2 cells.
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Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production.
Underhill DM, Rossnagle E, Lowell CA, Simmons RM.
Blood 2005 Jun; 106(7):2543.
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p47phox-Dependent Reactive Oxygen Species Stimulate Nuclear Translocation of the FoxO1 Transcription Factor During Metabolic Inhibition in Cardiomyoblasts.
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