NCF1 (phospho S345) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: TNF treated HepG2 cells Lane 2: antigen-specific peptide treated HepG2 cells with NCF1 (phospho S345) polyclonal antibody (Cat # PAB29250) at 1:500-1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by NCF1 (phospho S345) polyclonal antibody (Cat # PAB29250) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human NCF1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S345 of human NCF1.
Host
Rabbit
Theoretical MW (kDa)
45
Reactivity
Human
Specificity
This antibody detects endogenous levels of NCF1 only when phosphorylated at serine 345.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: TNF treated HepG2 cells Lane 2: antigen-specific peptide treated HepG2 cells with NCF1 (phospho S345) polyclonal antibody (Cat # PAB29250) at 1:500-1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by NCF1 (phospho S345) polyclonal antibody (Cat # PAB29250) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — NCF1
Entrez GeneID
653361Protein Accession#
P14598Gene Name
NCF1
Gene Alias
FLJ79451, NCF1A, NOXO2, SH3PXD1A, p47phox
Gene Description
neutrophil cytosolic factor 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq
Other Designations
NADPH oxidase organizer 2
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