EIF2S1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from A-431 cells, untreated or EGF-treated (200 ng/ml, 5 min), using EIF2S1 polyclonal antibody (Cat # PAB5339) .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EIF2S1.
Immunogen
A synthetic peptide corresponding to residues surrounding S51 of human EIF2S1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from A-431 cells, untreated or EGF-treated (200 ng/ml, 5 min), using EIF2S1 polyclonal antibody (Cat # PAB5339) .Immunohistochemistry
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Gene Info — EIF2S1
Entrez GeneID
1965Gene Name
EIF2S1
Gene Alias
EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A
Gene Description
eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Omim ID
603907Gene Ontology
HyperlinkGene Summary
The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM
Other Designations
eIF-2-alpha|eukaryotic translation initiation factor 2, subunit 1 (alpha, 35kD )
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Interactome
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Disease
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Publication Reference
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Identification of a hydrogen peroxide-induced PP1-JNK1-Sp1 signaling pathway for gene regulation.
Chu S, Ferro TJ.
American Journal of Physiology. Lung Cellular and Molecular Physiology 2006 Nov; 291(5):L983.
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Hypoxia inhibits protein synthesis through a 4E-BP1 and elongation factor 2 kinase pathway controlled by mTOR and uncoupled in breast cancer cells.
Connolly E, Braunstein S, Formenti S, Schneider RJ.
Molecular and Cellular Biology 2006 May; 26(10):3955.
Application：WB, Human, CRL2324, HTB20, MCF10A cells.
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Rapamycin-induced translational derepression of GCN4 mRNA involves a novel mechanism for activation of the eIF2 alpha kinase GCN2.
Kubota H, Obata T, Ota K, Sasaki T, Ito T.
The Journal of Biological Chemistry 2003 Jun; 278(23):20457.
Application：WB-Tr, Yeast, Yeast cells.
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Identification of a hydrogen peroxide-induced PP1-JNK1-Sp1 signaling pathway for gene regulation.
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