EIF2S1 recombinant monoclonal antibody, clone R07-5H9
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More Files
- More Functions
Western Blot
Western blot analysis of Phospho-eIF2A (Ser51) in K562, C6, 3T3, Hela lysates using Phospho-eIF2A (Ser51) antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EIF2S1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against synthetic phosphopeptide corresponding to residues surrounding Ser51 of human EIF2S1.
Theoretical MW (kDa)
Calculated MW: 36 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine pH 7.4 (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Phospho-eIF2A (Ser51) in K562, C6, 3T3, Hela lysates using Phospho-eIF2A (Ser51) antibody. -
Gene Info — EIF2S1
Entrez GeneID
1965Protein Accession#
P05198Gene Name
EIF2S1
Gene Alias
EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A
Gene Description
eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Omim ID
603907Gene Ontology
HyperlinkGene Summary
The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM
Other Designations
eIF-2-alpha|eukaryotic translation initiation factor 2, subunit 1 (alpha, 35kD )
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Interactome
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Disease
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