PDK2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of PDK2 polyclonal antibody (Cat # PAB4562) in mouse intestine tissue lysate (35 ug/lane). PDK2 (arrow) was detected using the purified PDK2 polyclonal antibody (Cat # PAB4562).
Western Blot (Transfected lysate)
Western blot analysis of PDK2 (arrow) using PDK2 polyclonal antibody (Cat # PAB4562). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK2 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PDK2 polyclonal antibody (Cat # PAB4562) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PDK2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 378-407 at C-terminus of human PDK2.
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of PDK2 polyclonal antibody (Cat # PAB4562) in mouse intestine tissue lysate (35 ug/lane). PDK2 (arrow) was detected using the purified PDK2 polyclonal antibody (Cat # PAB4562).Western Blot (Transfected lysate)
Western blot analysis of PDK2 (arrow) using PDK2 polyclonal antibody (Cat # PAB4562). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK2 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PDK2 polyclonal antibody (Cat # PAB4562) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PDK2
Entrez GeneID
5164Protein Accession#
NP_002602;Q15119Gene Name
PDK2
Gene Alias
PDHK2
Gene Description
pyruvate dehydrogenase kinase, isozyme 2
Omim ID
602525Gene Ontology
HyperlinkGene Summary
O
Other Designations
pyruvate dehydrogenase kinase, isoenzyme 2
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Interactome
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Publication Reference
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Fenofibrate suppresses growth of the human hepatocellular carcinoma cell via PPAR?-independent mechanisms.
Yamasaki D, Kawabe N, Nakamura H, Tachibana K, Ishimoto K, Tanaka T, Aburatani H, Sakai J, Hamakubo T, Kodama T, Doi T.
European Journal of Cell Biology 2011 Aug; 90(8):657.
Application：WB-Ce, Human, Huh-7 cells.
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Regulation of pyruvate dehydrogenase in the common killifish, Fundulus heteroclitus, during hypoxia exposure.
Richards JG, Sardella BA, Schulte PM.
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 2008 Jun; 295(3):R979.
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Estrogen-related receptors stimulate pyruvate dehydrogenase kinase isoform 4 gene expression.
Zhang Y, Ma K, Sadana P, Chowdhury F, Gaillard S, Wang F, McDonnell DP, Unterman TG, Elam MB, Park EA.
The Journal of Biological Chemistry 2006 Nov; 281(52):39897.
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Reperfusion-induced translocation of deltaPKC to cardiac mitochondria prevents pyruvate dehydrogenase reactivation.
Churchill EN, Murriel CL, Chen CH, Mochly-Rosen D, Szweda LI.
Circulation Research 2005 Jun; 97(1):78.
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Fenofibrate suppresses growth of the human hepatocellular carcinoma cell via PPAR?-independent mechanisms.
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