PRKCQ polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKCQ.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PRKCQ.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of PRKCQ polyclonal antibody (Cat # PAB4547) in placenta lysate . PRKCQ (Arrow) was detected using purified PRKCQ polyclonal antibody (Cat # PAB4547) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PRKCQ polyclonal antibody (Cat # PAB4547) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKCQ
Entrez GeneID
5588Protein Accession#
Q04759Gene Name
PRKCQ
Gene Alias
MGC126514, MGC141919, PRKCT, nPKC-theta
Gene Description
protein kinase C, theta
Omim ID
600448Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq
Other Designations
OTTHUMP00000019053|OTTHUMP00000043364|OTTHUMP00000043365
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Publication Reference
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Diacylglycerol-dependent binding recruits PKCtheta and RasGRP1 C1 domains to specific subcellular localizations in living T lymphocytes.
Carrasco S, Merida I.
Molecular Biology of the Cell 2004 Jun; 15(6):2932.
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Akt mediates insulin-stimulated phosphorylation of Ndrg2: evidence for cross-talk with protein kinase C theta.
Burchfield JG, Lennard AJ, Narasimhan S, Hughes WE, Wasinger VC, Corthals GL, Okuda T, Kondoh H, Biden TJ, Schmitz-Peiffer C.
The Journal of Biological Chemistry 2004 Feb; 279(18):18623.
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Association of CBFA2 mutation with decreased platelet PKC-theta and impaired receptor-mediated activation of GPIIb-IIIa and pleckstrin phosphorylation: proteins regulated by CBFA2 play a role in GPIIb-IIIa activation.
Sun L, Mao G, Rao AK.
Blood 2003 Oct; 103(3):948.
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Diacylglycerol-dependent binding recruits PKCtheta and RasGRP1 C1 domains to specific subcellular localizations in living T lymphocytes.
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