AURKC polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The AURKC polyclonal antibody (Cat # PAB4511) is used in Western blot to detect AURKC in lysates of 293 cells expressing Flag tag (lane 1) , Flag-tagged AURKA (lane 2) , Flag-tagged AURKB (lane 3) or Flag-tagged AURKC (lane 4) .
Immunofluorescence
In the immunofluorescence experiment, staining of HeLa cells expressing GFP-AURKC is performed at different cellular mitotic stages with The AURKC polyclonal antibody (Cat # PAB4511) as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-AURKC merged to DAPI staining (column D). Data is kindly provided by Drs . K . Sasai and S . Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AURKC.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human AURKC.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunofluorescence (1:100-500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The AURKC polyclonal antibody (Cat # PAB4511) is used in Western blot to detect AURKC in lysates of 293 cells expressing Flag tag (lane 1) , Flag-tagged AURKA (lane 2) , Flag-tagged AURKB (lane 3) or Flag-tagged AURKC (lane 4) .Immunofluorescence
In the immunofluorescence experiment, staining of HeLa cells expressing GFP-AURKC is performed at different cellular mitotic stages with The AURKC polyclonal antibody (Cat # PAB4511) as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-AURKC merged to DAPI staining (column D). Data is kindly provided by Drs . K . Sasai and S . Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).Enzyme-linked Immunoabsorbent Assay
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Gene Info — AURKC
Entrez GeneID
6795Protein Accession#
Q9UQB9Gene Name
AURKC
Gene Alias
AIE2, AIK3, AurC, STK13, aurora-C
Gene Description
aurora kinase C
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
aurora/IPL1-related kinase 3|aurora/IPL1/EG2 protein 2|serine/threonine kinase 13 (aurora/IPL1-like)
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Interactome
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Disease
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Publication Reference
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Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3.
Kimura M, Matsuda Y, Yoshioka T, Okano Y.
The Journal of Biological Chemistry 1999 Mar; 274(11):7334.
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Cloning of STK13, a third human protein kinase related to Drosophila aurora and budding yeast Ipl1 that maps on chromosome 19q13.3-ter.
Bernard M, Sanseau P, Henry C, Couturier A, Prigent C.
Genomics 1998 Nov; 53(3):406.
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Protein kinase profile of sperm and eggs: cloning and characterization of two novel testis-specific protein kinases (AIE1, AIE2) related to yeast and fly chromosome segregation regulators.
Tseng TC, Chen SH, Hsu YP, Tang TK.
DNA and Cell Biology 1998 Oct; 17(10):823.
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Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3.
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