EZH1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of 293 cell lysate (35 ug/lane) with EZH1 polyclonal antibody (Cat # PAB4353).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with EZH1 polyclonal antibody (Cat # PAB4353) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EZH1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human EZH1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 293 cell lysate (35 ug/lane) with EZH1 polyclonal antibody (Cat # PAB4353).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with EZH1 polyclonal antibody (Cat # PAB4353) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — EZH1
Entrez GeneID
2145Protein Accession#
Q92800Gene Name
EZH1
Gene Alias
KIAA0388
Gene Description
enhancer of zeste homolog 1 (Drosophila)
Omim ID
601674Gene Ontology
HyperlinkGene Summary
EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM
Other Designations
enhancer of zeste homolog 1
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Interactome
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Disease
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Publication Reference
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Cloning and expression of a human/mouse Polycomb group gene, ENX-2/Enx-2.
Ogawa M, Hiraoka Y, Taniguchi K, Aiso S.
Biochimica et Biophysica Acta 1998 Jan; 1395(2):151.
Application：ICC, Mouse, Neuro-2a cells.
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Characterization of EZH1, a human homolog of Drosophila Enhancer of zeste near BRCA1.
Abel KJ, Brody LC, Valdes JM, Erdos MR, McKinley DR, Castilla LH, Merajver SD, Couch FJ, Friedman LS, Ostermeyer EA, Lynch ED, King MC, Welcsh PL, Osborne-Lawrence S, Spillman M, Bowcock AM, Collins FS, Weber BL.
Genomics 1996 Oct; 37(2):161.
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22 genes from chromosome 17q21: cloning, sequencing, and characterization of mutations in breast cancer families and tumors.
Friedman LS, Ostermeyer EA, Lynch ED, Welcsh P, Szabo CI, Meza JE, Anderson LA, Dowd P, Lee MK, Rowell SE, et al..
Genomics 1995 Jan; 25(1):256.
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Cloning and expression of a human/mouse Polycomb group gene, ENX-2/Enx-2.
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