FDFT1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of FDFT1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human FDFT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Immunohistochemistry (1:50-100)
Western Blot (1:50-200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of FDFT1 polyclonal antibody (Cat # PAB4321) in A-375 cell line lysate . FDFT1 (arrow) was detected using the purified FDFT1 polyclonal antibody (Cat # PAB4321) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with FDFT1 polyclonal antibody (Cat # PAB4321) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — FDFT1
Entrez GeneID
2222Protein Accession#
FDFT_HUMANGene Name
FDFT1
Gene Alias
DGPT, ERG9, SQS, SS
Gene Description
farnesyl-diphosphate farnesyltransferase 1
Omim ID
184420Gene Ontology
HyperlinkGene Summary
This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. [provided by RefSeq
Other Designations
FPP:FPP farnesyltransferase|presqualene-di-diphosphate synthase|squalene synthase
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Interactome
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Pathway
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Disease
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Publication Reference
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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA,
PNAS 2002 Dec; 99(26):16899.
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Expression, purification, and characterization of the human squalene synthase: use of yeast and baculoviral systems.
Soltis DA, McMahon G, Caplan SL, Dudas DA, Chamberlin HA, Vattay A, Dottavio D, Rucker ML, Engstrom RG, Cornell-Kennon SA, et al..
Archives of Biochemistry and Biophysics 1995 Feb; 316(2):713.
Application:WB-Re, WB-Tr, Insect, Yeast, Recombinant proteins, Sf9 cells, Yeast cells.
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Transcriptional regulation by lovastatin and 25-hydroxycholesterol in HepG2 cells and molecular cloning and expression of the cDNA for the human hepatic squalene synthase.
Jiang G, McKenzie TL, Conrad DG, Shechter I.
The Journal of Biological Chemistry 1993 Jun; 268(17):12818.
Application:WB-Tr, Human, HepG2 cells.
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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
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