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Last updated: 2017/2/26
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USP14 polyclonal antibody

  • Catalog # : PAB4199
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of USP14.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human USP14.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:100-500)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The USP14 polyclonal antibody (Cat # PAB4199) is used in Western blot to detect USP14 in HeLa cell lysates . Transfection data is kindly provided by Dr . B . Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland) .
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The USP14 polyclonal antibody (Cat # PAB4199) is used in Western blot to detect USP14 in A-375 . Transfection data is kindly provided by Dr . B . Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland) .
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with USP14 polyclonal antibody (Cat # PAB4199) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 9097
  • Gene Name:
  • USP14
  • Gene Alias:
  • TGT
  • Gene Description:
  • ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
  • Gene Summary:
  • This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
  • Other Designations:
  • deubiquitinating enzyme 14,tRNA-guanine transglycosylase, 60-kD subunit,ubiquitin carboxyl-terminal hydrolase 14,ubiquitin specific protease 14,ubiquitin specific protease 14 (tRNA-guanine transglycosylase),ubiquitin thiolesterase 14,ubiquitin-specific pr
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