PDGFRL polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate (35 ug/lane) with PDGFRL polyclonal antibody (Cat # PAB3378).
Western Blot (Transfected lysate)
Western blot analysis of PDGFRL (arrow) using PDGFRL polyclonal antibody (Cat # PAB3378).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDGFRL gene (Lane 2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PDGFRL polyclonal antibody (Cat # PAB3378) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PDGFRL.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PDGFRL.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate (35 ug/lane) with PDGFRL polyclonal antibody (Cat # PAB3378).Western Blot (Transfected lysate)
Western blot analysis of PDGFRL (arrow) using PDGFRL polyclonal antibody (Cat # PAB3378).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDGFRL gene (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PDGFRL polyclonal antibody (Cat # PAB3378) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — PDGFRL
Entrez GeneID
5157Protein Accession#
Q15198Gene Name
PDGFRL
Gene Alias
PDGRL, PRLTS
Gene Description
platelet-derived growth factor receptor-like
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein with significant sequence similarity to the ligand binding domain of platelet-derived growth factor receptor beta. Mutations in this gene, or deletion of a chromosomal segment containing this gene, are associated with sporadic hepatocellular carcinomas, colorectal cancers, and non-small cell lung cancers. This suggests this gene product may function as a tumor suppressor. [provided by RefSeq
Other Designations
OTTHUMP00000161390|platelet-derived growth factor receptor-like protein|platelet-derived growth factor-beta-like tumor suppressor
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