NEK7 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NEK7.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human NEK7.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of NEK7 (arrow) using NEK7 polyclonal antibody (Cat # PAB3288).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NEK7 gene (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEK7 polyclonal antibody (Cat # PAB3288) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — NEK7
Entrez GeneID
140609Protein Accession#
Q8TDX7Gene Name
NEK7
Gene Alias
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Gene Description
NIMA (never in mitosis gene a)-related kinase 7
Omim ID
606848Gene Ontology
HyperlinkGene Summary
NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM
Other Designations
OTTHUMP00000033680
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Interactome
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Disease
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Publication Reference
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A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases.
Belham C, Roig J, Caldwell JA, Aoyama Y, Kemp BE, Comb M, Avruch J.
The Journal of Biological Chemistry 2003 Sep; 278(37):34897.
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Identification and assignment of the human NIMA-related protein kinase 7 gene (NEK7) to human chromosome 1q31.3.
Kimura M, Okano Y.
Cytogenetics and Cell Genetics 2001 Dec; 94(1-2):33.
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A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases.
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