BRD3 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BRD3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human BRD3.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
The BRD3 polyclonal antibody (Cat # PAB3246) is used in Western blot to detect BRD3 in mouse brain tissue lysate (Lane 1) and Sacs-2 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with BRD3 polyclonal antibody (Cat # PAB3246) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — BRD3
Entrez GeneID
8019Protein Accession#
Q15059Gene Name
BRD3
Gene Alias
FLJ23227, FLJ41328, KIAA0043, ORFX, RING3L
Gene Description
bromodomain containing 3
Omim ID
601541Gene Ontology
HyperlinkGene Summary
This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. [provided by RefSeq
Other Designations
OTTHUMP00000022724|RING3-like|bromodomain containing protein 3|bromodomain-containing 3
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Interactomes
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Publication Reference
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Chromosomal localization, gene structure and transcription pattern of the ORFX gene, a homologue of the MHC-linked RING3 gene.
Thorpe KL, Gorman P, Thomas C, Sheer D, Trowsdale J, Beck S.
Gene 1997 Oct; 200(1-2):177.
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Phylogeny and structure of the RING3 gene.
Thorpe KL, Abdulla S, Kaufman J, Trowsdale J, Beck S.
Immunogenetics 1996 Sep; 44(5):391.
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Chromosomal localization, gene structure and transcription pattern of the ORFX gene, a homologue of the MHC-linked RING3 gene.
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