CRBN polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human CRBN.
Sequence
EVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQERE
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody reacts to CRBN.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (0.25-2 ug/mL)
Immunohistochemistry (1:200 - 1:500)
Western Blot (0.04-0.4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of mouse cell lines NIH-3T3 and rat cell line NBT-II.Western Blot (Transfected lysate)
Western blot analysis of HEK293 cells transfected with control siRNA, target specific siRNA probe # 1, using CRBN polyclonal antibody (Cat # PAB31825). Loading control: Anti-GAPDH.Immunohistochemistry
Immunohistochemical staining of human testis shows strong positivity in cells in seminiferous ducts.Immunohistochemistry
Immunohistochemical staining of human colon shows moderate cytoplasmic and nuclear positivity in peripheral ganglion.Immunohistochemistry
Immunohistochemical staining of human skeletal muscle shows only weak cytoplasmic positivity in striated muscle fibers.Immunohistochemistry
Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli. -
Gene Info — CRBN
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Interactome
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