Goat Fab Anti-Human IgG (H&L) secondary antibody (FITC)
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More Files
- More Functions
Western Blot
Western Blot analysis human IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1000 for 60 min at RT. Blocking: for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for human IgG.
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Specification
Product Description
Goat Fab polyclonal antibody raised against human IgG (H&L). The antibody is conjugated with Fluorescein (FITC).
Immunogen
Human IgG whole molecule.
Host
Goat
Reactivity
Human
Specificity
IgG (H&L). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein and anti-goat serum. No reaction was observed against anti-papain or anti-goat IgG F(c).
Form
Lyophilized
Conjugation
FITC
Purification
This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
Isotype
IgG Fab
Conjugation Note
FP Value: 3.49 moles Fluorescein (FITC) per mole of IgG Fab.
Recommend Usage
FLISA (1:10000-1:50000)
Flow Cytometry (1:500-1:2500)
Immunofluorescence (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) sodium azide).
Storage Instruction
Store vial at 4°C prior to restoration.
After reconstitution with 1.0 mL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis human IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1000 for 60 min at RT. Blocking: for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for human IgG.Immunofluorescence
Flow Cytometry
Fluorescence-linked Immunosorbent Assay
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