SEMA7A polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human SEMA7A.
Immunogen
Recombinant protein corresponding to human SEMA7A.
Sequence
QDRVDFGQTEPHTVLFHEPGSSSVWVGGRGKVYLFDFPEGKNASVRTVNIGSTKGSCLDKRDCENYITLLERRSEGLLACGTNARHPSCWNLVNGTVVPLGEMRGYAPFSPDENSLVLFEGD
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with SEMA7A polyclonal antibody (Cat # PAB31251).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human duodenum with SEMA7A polyclonal antibody (Cat # PAB31251) shows distinct cytoplasmic and membranous positivity in glandular cells. -
Gene Info — SEMA7A
Entrez GeneID
8482Protein Accession#
O75326Gene Name
SEMA7A
Gene Alias
CD108, CDw108, H-SEMA-K1, H-Sema-L, JMH, MGC126692, MGC126696, SEMAK1, SEMAL
Gene Description
semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
Omim ID
607961Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene binds to cell surfaces through a glycosylphosphatidylinositol (GPI) linkage. The encoded glycoprotein is found on activated lymphocytes and erythrocytes. This protein may be involved in immunomodulatory and neuronal processes. Defects in this gene can result in loss of bone mineral density (BMD). Three transcript variants encoding different isoforms have been found for this gene
Other Designations
John Milton Hagen blood group H-Sema K1|sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (JMH blood group)|sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A|semaphorin 7A|semaphorin K1|semaphorin L
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