MAP2K1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells) and Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) with MAP2K1 polyclonal antibody (Cat # PAB30869).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human hippocampus with MAP2K1 polyclonal antibody (Cat # PAB30869) shows moderate cytoplasmic positivity in neuronal cells.
Immunofluorescence
Immunofluorescent staining of A-431 cells with MAP2K1 polyclonal antibody (Cat # PAB30869) (Green) shows positivity in plasma membrane and cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human MAP2K1.
Immunogen
Recombinant protein corresponding to human MAP2K1.
Sequence
MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQ
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:50)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells) and Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) with MAP2K1 polyclonal antibody (Cat # PAB30869).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human hippocampus with MAP2K1 polyclonal antibody (Cat # PAB30869) shows moderate cytoplasmic positivity in neuronal cells.Immunofluorescence
Immunofluorescent staining of A-431 cells with MAP2K1 polyclonal antibody (Cat # PAB30869) (Green) shows positivity in plasma membrane and cytoplasm. -
Gene Info — MAP2K1
Entrez GeneID
5604Protein Accession#
Q02750Gene Name
MAP2K1
Gene Alias
MAPKK1, MEK1, MKK1, PRKMK1
Gene Description
mitogen-activated protein kinase kinase 1
Omim ID
176872Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq
Other Designations
protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
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Interactome
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Pathway
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Disease
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Publication Reference
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MEK1 is associated with carboplatin resistance and is a prognostic biomarker in epithelial ovarian cancer.
Pénzváltó Z, Lánczky A, Lénárt J, Meggyesházi N, Krenács T, Szoboszlai N, Denkert C, Pete I, Győrffy B.
BMC Cancer 2014 Nov; 14:837.
Application：IHC-P, Human, Human epithelial ovarian cancer.
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MEK1 is associated with carboplatin resistance and is a prognostic biomarker in epithelial ovarian cancer.
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