Immunofluorescence (1-4 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500) Western Blot (1:100-1:250) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with KIF11 polyclonal antibody (Cat # PAB30770).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human duodenum with KIF11 polyclonal antibody (Cat # PAB30770) shows strong nuclear, membranous and cytoplasmic positivity in glandular cells.
Immunofluorescent staining of U-2 OS with KIF11 polyclonal antibody (Cat # PAB30770) (Green) shows positivity in cytoplasm.
This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq
OTTHUMP00000020099,kinesin-like 1,kinesin-like spindle protein,kinesin-related motor protein,thyroid receptor interacting protein 5