KIF11 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with KIF11 polyclonal antibody (Cat # PAB30770).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human duodenum with KIF11 polyclonal antibody (Cat # PAB30770) shows strong nuclear, membranous and cytoplasmic positivity in glandular cells.
Immunofluorescence
Immunofluorescent staining of U-2 OS with KIF11 polyclonal antibody (Cat # PAB30770) (Green) shows positivity in cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human KIF11.
Immunogen
Recombinant protein corresponding to human KIF11.
Sequence
EMILSPTVVSILKINSQLKHIFKTSLTVADKIEDQKKELDGFLSILCNNLHELQENTICSLVESQKQCGNLTEDLKTIKQTHSQELCKLMNLWTERFCALEEKCENIQKPLSSVQENIQQKSKDIVNKMTFHSQKFCADSDGFSQELR
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with KIF11 polyclonal antibody (Cat # PAB30770).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human duodenum with KIF11 polyclonal antibody (Cat # PAB30770) shows strong nuclear, membranous and cytoplasmic positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of U-2 OS with KIF11 polyclonal antibody (Cat # PAB30770) (Green) shows positivity in cytoplasm. -
Gene Info — KIF11
Entrez GeneID
3832Protein Accession#
P52732Gene Name
KIF11
Gene Alias
EG5, HKSP, KNSL1, TRIP5
Gene Description
kinesin family member 11
Omim ID
148760Gene Ontology
HyperlinkGene Summary
This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq
Other Designations
OTTHUMP00000020099|kinesin-like 1|kinesin-like spindle protein|kinesin-related motor protein|thyroid receptor interacting protein 5
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Interactome
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Disease
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