TPX2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human TPX2.
Immunogen
Recombinant protein corresponding to human TPX2.
Sequence
QELEKSMKMQQEVVEMRKKNEEFKKLALAGIGQPVKKSVSQVTKSVDFHFRTDERIKQHPKNQEEYKEVNFTSELRKHPSSPARVTKGCTIVKPFNLSQGKKRTFDETVSTYVPLAQQVEDFHKRTPNRYHLRSKKD
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with TPX2 polyclonal antibody (Cat # PAB30737).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human bone marrow with TPX2 polyclonal antibody (Cat # PAB30737) shows strong nuclear positivity in bone marrow poietic cells.Immunofluorescence
Immunofluorescent staining of U-2 OS with TPX2 polyclonal antibody (Cat # PAB30737) (Green) shows positivity in nucleus but excluded from the nucleoli. -
Gene Info — TPX2
Entrez GeneID
22974Protein Accession#
Q9ULW0Gene Name
TPX2
Gene Alias
C20orf1, C20orf2, DIL-2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, REPP86, p100
Gene Description
TPX2, microtubule-associated, homolog (Xenopus laevis)
Omim ID
605917Gene Ontology
HyperlinkGene Summary
microtubule-associated
Other Designations
TPX2, microtubule-associated protein homolog|differentially expressed in lung cells|hepatocellular carcinoma-associated antigen 519|preferentially expressed in colorectal cancer|restricted expression proliferation associated protein 100|targeting protein
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Interactome
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Disease
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