MEF2D polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with MEF2D polyclonal antibody (Cat # PAB30710).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymph node with MEF2D polyclonal antibody (Cat # PAB30710) shows strong nuclear positivity in reaction center cells and lymphoid cells outside reaction center.
Immunofluorescence
Immunofluorescent staining of U-2 OS with MEF2D polyclonal antibody (Cat # PAB30710) (Green) shows positivity in vesicles and nucleus but excluded from the nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human MEF2D.
Immunogen
Recombinant protein corresponding to human MEF2D.
Sequence
VPVSNQSSLQFSNPSGSLVTPSLVTSSLTDPRLLSPQQPALQRNSVSPGLPQRPASAGAMLGGDLNSANGACPSPVGNGYVSARASPGLLPVANGNSLNKVIPAKSPPPPTHSTQLGAPSRKPDLRVITSQAG
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with MEF2D polyclonal antibody (Cat # PAB30710).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymph node with MEF2D polyclonal antibody (Cat # PAB30710) shows strong nuclear positivity in reaction center cells and lymphoid cells outside reaction center.Immunofluorescence
Immunofluorescent staining of U-2 OS with MEF2D polyclonal antibody (Cat # PAB30710) (Green) shows positivity in vesicles and nucleus but excluded from the nucleoli. -
Gene Info — MEF2D
Entrez GeneID
4209Protein Accession#
Q14814Gene Name
MEF2D
Gene Alias
DKFZp686I1536
Gene Description
myocyte enhancer factor 2D
Omim ID
600663Gene Ontology
HyperlinkGene Summary
polypeptide D (myocyte enhancer factor 2D)|MEF2D/DAZAP1 fusion|OTTHUMP00000031810|myocyte enhancer factor 2D/deleted in azoospermia associated protein 1 fusion protein
Other Designations
MADS box transcription enhancer factor 2, polypeptide D (myocyte enhancer factor 2D)|MEF2D/DAZAP1 fusion|OTTHUMP00000031810|myocyte enhancer factor 2D/deleted in azoospermia associated protein 1 fusion protein
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Interactome
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Disease
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