Immunofluorescence (1-4 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500) Western Blot (1:100-1:250) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with MEF2D polyclonal antibody (Cat # PAB30710).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymph node with MEF2D polyclonal antibody (Cat # PAB30710) shows strong nuclear positivity in reaction center cells and lymphoid cells outside reaction center.
Immunofluorescent staining of U-2 OS with MEF2D polyclonal antibody (Cat # PAB30710) (Green) shows positivity in vesicles and nucleus but excluded from the nucleoli.