MCM7 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells) and Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) with MCM7 polyclonal antibody (Cat # PAB30650).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human placenta with MCM7 polyclonal antibody (Cat # PAB30650) shows strong nuclear positivity in trophoblastic cells.
Immunofluorescence
Immunofluorescent staining of MCF7 cells with MCM7 polyclonal antibody (Cat # PAB30650) (Green) shows positivity in nucleus but excluded from the nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human MCM7.
Immunogen
Recombinant protein corresponding to human MCM7.
Sequence
QSPTFMPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEAHRIVKMNKSEDDESGAGE
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells) and Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) with MCM7 polyclonal antibody (Cat # PAB30650).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human placenta with MCM7 polyclonal antibody (Cat # PAB30650) shows strong nuclear positivity in trophoblastic cells.Immunofluorescence
Immunofluorescent staining of MCF7 cells with MCM7 polyclonal antibody (Cat # PAB30650) (Green) shows positivity in nucleus but excluded from the nucleoli. -
Gene Info — MCM7
Entrez GeneID
4176Protein Accession#
P33993Gene Name
MCM7
Gene Alias
CDABP0042, CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS-146
Gene Description
minichromosome maintenance complex component 7
Omim ID
600592Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
DNA replication licensing factor MCM7|MCM7 minichromosome maintenance deficient 7|homolog of S. cerevisiae Cdc47|minichromosome maintenance deficient 7
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Interactome
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Pathway
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Disease
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