Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach with ATG10 polyclonal antibody (Cat # PAB30243) shows moderate membranous and cytoplasmic positivity in glandular cells at 1:50 - 1:200 dilution.
Immunofluorescent staining of human cell line MCF-7 with ATG10 polyclonal antibody (Cat # PAB30243) shows positivity in nucleus and nucleoli under 1-4 ug/mL concentration. Antibody staining is shown in green.
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM