CHAF1B polyclonal antibody
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More Files
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of human CHAF1B.
Immunogen
A synthetic peptide corresponding to C-terminus of human CHAF1B.
Sequence
PPSSVPTSVISTPSTEEIQSETPGDAQGSPPELKRPRLDENKGGTESLDP
Host
Rabbit
Theoretical MW (kDa)
61
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-8 ug/mL)
Western Blot (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (2% sucrose, 0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Jurkat cell lysate with CHAF1B polyclonal antibody (Cat # PAB29994) at 5 ug/mL working concentration.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney with CHAF1B polyclonal antibody (Cat # PAB29994) at 4-8 ug/mL working concentration. -
Gene Info — CHAF1B
Entrez GeneID
8208GeneBank Accession#
NM_005441Protein Accession#
NP_005432;Q13112Gene Name
CHAF1B
Gene Alias
CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7
Gene Description
chromatin assembly factor 1, subunit B (p60)
Omim ID
601245Gene Ontology
HyperlinkGene Summary
Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq
Other Designations
CAF-1 subunit B|CAF-I 60 kDa subunit|M-phase phosphoprotein 7|chromatin assembly factor 1 subunit B|chromatin assembly factor I p60 subunit|human chromatin assembly factor-I p60 subunit
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