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BIK (phospho T33) polyclonal antibody

  • Catalog # : PAB29645
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human BIK.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T33 of human BIK.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 30
  • Reactivity:
  • Human
  • Specificity:
  • BIK (phospho T33) polyclonal antibody detects endogenous levels of human BIK only when phosphorylated at threonine 33.
  • Form:
  • Liquid
  • Purification:
  • Affinity chromatography
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: Untreated K562 cell lysates, Lane 2: Synthesized peptide treated K562 cell lysates reacted with BIK (phospho T33) polyclonal antibody (Cat # PAB29645) at 1:500-1:3000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded human lung carcinoma tissue reacted with BIK (phospho T33) polyclonal antibody (Cat # PAB29645) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 638
  • Gene Name:
  • BIK
  • Gene Alias:
  • BIP1,BP4,NBK
  • Gene Description:
  • BCL2-interacting killer (apoptosis-inducing)
  • Gene Summary:
  • The protein encoded by this gene is known to interact with cellular and viral survival-promoting proteins, such as BCL2 and the Epstein-Barr virus in order to enhance programed cell death. Because its activity is suppressed in the presence of survival-promoting proteins, this protein is suggested as a likely target for antiapoptotic proteins. This protein shares a critical BH3 domain with other death-promoting proteins, BAX and BAK. [provided by RefSeq
  • Other Designations:
  • BCL2-interacting killer,OTTHUMP00000028974,apoptosis-inducing NBK
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