BIK (phospho T33) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated K562 cell lysates, Lane 2: Synthesized peptide treated K562 cell lysates reacted with BIK (phospho T33) polyclonal antibody (Cat # PAB29645) at 1:500-1:3000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human lung carcinoma tissue reacted with BIK (phospho T33) polyclonal antibody (Cat # PAB29645) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human BIK.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T33 of human BIK.
Host
Rabbit
Theoretical MW (kDa)
30
Reactivity
Human
Specificity
BIK (phospho T33) polyclonal antibody detects endogenous levels of human BIK only when phosphorylated at threonine 33.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated K562 cell lysates, Lane 2: Synthesized peptide treated K562 cell lysates reacted with BIK (phospho T33) polyclonal antibody (Cat # PAB29645) at 1:500-1:3000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human lung carcinoma tissue reacted with BIK (phospho T33) polyclonal antibody (Cat # PAB29645) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide. -
Gene Info — BIK
Entrez GeneID
638Protein Accession#
Q13323Gene Name
BIK
Gene Alias
BIP1, BP4, NBK
Gene Description
BCL2-interacting killer (apoptosis-inducing)
Omim ID
603392Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is known to interact with cellular and viral survival-promoting proteins, such as BCL2 and the Epstein-Barr virus in order to enhance programed cell death. Because its activity is suppressed in the presence of survival-promoting proteins, this protein is suggested as a likely target for antiapoptotic proteins. This protein shares a critical BH3 domain with other death-promoting proteins, BAX and BAK. [provided by RefSeq
Other Designations
BCL2-interacting killer|OTTHUMP00000028974|apoptosis-inducing NBK
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Interactome
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Disease
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