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MAPT (phospho S404) polyclonal antibody

  • Catalog # : PAB29610
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human MAPT.
  • Immunogen:
  • A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S404 of human MAPT.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 48, 62, 78
  • Reactivity:
  • Human, Mouse, Rabbit
  • Specificity:
  • MAPT (phospho S404) polyclonal antibody detects endogenous level of MAPT only when phosphorylated at serine 404.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunofluorescence (1:100-1:200)
    Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: C6 cells, Lane 2: calf intestinal phosphatase (CIP) treated C6 cells with MAPT (phospho S404) polyclonal antibody (Cat# PAB29610) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of rat hippocampal region tissue from a model with Alzheimer using MAPT (phospho S404) polyclonal antibody (Cat# PAB29610) under 1:50-1:100 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of methanol-fixed Hela cells using MAPT (phospho S404) polyclonal antibody (Cat# PAB29610) at 1:100-1:200 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4137
  • Gene Name:
  • MAPT
  • Gene Alias:
  • DDPAC,FLJ31424,FTDP-17,MAPTL,MGC138549,MSTD,MTBT1,MTBT2,PPND,TAU
  • Gene Description:
  • microtubule-associated protein tau
  • Gene Summary:
  • This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq
  • Other Designations:
  • G protein beta1/gamma2 subunit-interacting factor 1,microtubule-associated protein tau, isoform 4
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