NUDC (phospho S326) polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human NUDC.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S326 of human NUDC.
Host
Rabbit
Theoretical MW (kDa)
45
Reactivity
Human, Mouse, Rat
Specificity
NUDC (phospho S326) polyclonal antibody detects endogenous levels of human NUDC only when phosphorylated at serine 326.
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Synthesized peptide treated HepG2 cells, Lane 2: HepG2 cells with NUDC (phospho S326) polyclonal antibody (Cat # PAB29584) at 1:500-1:3000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast carcinoma tissue with NUDC (phospho S326) polyclonal antibody (Cat # PAB29584) under 1:50-1:100 dilution (A) or treated with synthesized peptide (B). -
Gene Info — NUDC
Entrez GeneID
10726Protein Accession#
Q9Y266Gene Name
NUDC
Gene Alias
HNUDC, MNUDC, NPD011
Gene Description
nuclear distribution gene C homolog (A. nidulans)
Omim ID
610325Gene Ontology
HyperlinkGene Summary
NudC was first identified as a regulator of nuclear movement in the asexual reproductive cycle of the filamentous fungus Aspergillus nidulans. Human NUDC is a nuclear movement protein that associates with dynein (see DYNC1H1; MIM 600112) (Aumais et al., 2003 [PubMed 12679384]).[supplied by OMIM
Other Designations
OTTHUMP00000004405|nuclear distribution gene C homolog
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