Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach with CDC6 polyclonal antibody (Cat# PAB29498) shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunofluorescent staining of HEK-293 cells with CDC6 polyclonal antibody (Cat# PAB29498) under 1-4 ug/mL working concentration shows positivity in cytoplasm, the Golgi apparatus and nucleus but excluded from the nucleoli. Antibody staining is shown in green.
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq