Immunohistochemical staining of human cerebellum with KPNA1 polyclonal antibody (Cat # PAB29452) shows moderate nuclear positivity in Purkinje cells at 1:500-1:1000 dilution.
Immunofluorescent staining of human cell line U-2 OS with KPNA1 polyclonal antibody (Cat # PAB29452) at 1-4 ug/mL concentration shows positivity in cytoplasm and nucleus but excluded from the nucleoli.
Recombination activating proteins RAG1 and RAG2 regulate and mediate V(D)J recombination, the process by which genes for immunoglobulins and T-cell receptors are generated. Several other ubiquitously expressed proteins are thought to be recruited in the recombination process. Among these are the genes affected in severe combined immune deficiency and genes involved in ds-DNA break repair. The protein encoded by this gene interacts with RAG1 and may play a role in V(D)J recombination. Two transcript variants, one protein-coding and the other not, have been found for this gene. [provided by RefSeq