LIMA1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human LIMA1.
Immunogen
Recombinant protein corresponding to human LIMA1.
Sequence
RPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGI
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:500-1:1000)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Human cell line RT-4 with LIMA1 polyclonal antibody (Cat # PAB29449) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with LIMA1 polyclonal antibody (Cat # PAB29449) shows strong membranous positivity in cells in tubules at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with LIMA1 polyclonal antibody (Cat # PAB29449) at 1-4 ug/mL concentration shows positivity in plasma membrane, actin filaments and focal adhesion sites. -
Gene Info — LIMA1
Entrez GeneID
51474Gene Name
LIMA1
Gene Alias
EPLIN, FLJ38853, MGC131726, SREBP3
Gene Description
LIM domain and actin binding 1
Omim ID
608364Gene Ontology
HyperlinkGene Summary
EPLIN is a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles (Maul et al., 2003 [PubMed 12566430]).[supplied by OMIM
Other Designations
1110021C24Rik|epithelial protein lost in neoplasm beta|sterol regulatory element binding protein 3
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