Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate) Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with GZMB polyclonal antibody (Cat # PAB29409) at 1:100-1:250 dilution.
Immunohistochemical staining of human lymph node with GZMB polyclonal antibody (Cat # PAB29409) shows strong cytoplasmic positivity in a subset of lymphoid cells outside reaction center at 1:50-1:200 dilution.
Immunofluorescent staining of human cell line U-2 OS with GZMB polyclonal antibody (Cat # PAB29409) at 1-4 ug/mL concentration shows positivity in vesicles.
Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq