VIM polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with VIM polyclonal antibody (Cat # PAB29375) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human duodenum with VIM polyclonal antibody (Cat # PAB29375) shows distinct positivity in mesenchymal and lymphoid cells at 1:1000-1:2500 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with VIM polyclonal antibody (Cat # PAB29375) at 1-4 ug/mL concentration shows positivity in intermediate filaments.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human VIM.
Immunogen
Recombinant protein corresponding to human VIM.
Sequence
FANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVES
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:1000-1:2500)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with VIM polyclonal antibody (Cat # PAB29375) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human duodenum with VIM polyclonal antibody (Cat # PAB29375) shows distinct positivity in mesenchymal and lymphoid cells at 1:1000-1:2500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with VIM polyclonal antibody (Cat # PAB29375) at 1-4 ug/mL concentration shows positivity in intermediate filaments. -
Gene Info — VIM
Entrez GeneID
7431Gene Name
VIM
Gene Alias
FLJ36605
Gene Description
vimentin
Omim ID
193060Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
Other Designations
OTTHUMP00000019224
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Interactome
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Disease
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