FHL1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with FHL1 polyclonal antibody (Cat # PAB29356) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human skeletal muscle with FHL1 polyclonal antibody (Cat # PAB29356) shows strong nuclear and cytoplasmic positivity in myocytes at 1:200-1:500 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with FHL1 polyclonal antibody (Cat # PAB29356) at 1-4 ug/mL concentration shows positivity in cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human FHL1.
Immunogen
Recombinant protein corresponding to human FHL1.
Sequence
KFCANTCVECRKPIGADSKEVHYKNRFWHDTCFRCAKCLHPLANETFVAKDNKILCNKCTTREDSPKCKGCFKAIVAGDQNVEYKGTVWHKDCFTCSNCKQV
Host
Rabbit
Reactivity
Human, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with FHL1 polyclonal antibody (Cat # PAB29356) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human skeletal muscle with FHL1 polyclonal antibody (Cat # PAB29356) shows strong nuclear and cytoplasmic positivity in myocytes at 1:200-1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with FHL1 polyclonal antibody (Cat # PAB29356) at 1-4 ug/mL concentration shows positivity in cytoplasm. -
Gene Info — FHL1
Entrez GeneID
2273Gene Name
FHL1
Gene Alias
FHL1B, FLH1A, KYO-T, MGC111107, SLIM1, XMPMA, bA535K18.1
Gene Description
four and a half LIM domains 1
Omim ID
300163Gene Ontology
HyperlinkGene Summary
LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM
Other Designations
Four-and-a-half LIM domains 1|OTTHUMP00000024097|bA535K18.1 (LIM protein SLIMMER)
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Interactome
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