EIF4ENIF1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Human cell line RT-4; Lane 2: Human cell line U-251MG sp; Lane 3: Human plasma (IgG/HSA depleted) with EIF4ENIF1 polyclonal antibody (Cat# PAB29326) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human pancreas with EIF4ENIF1 polyclonal antibody (Cat# PAB29326) shows strong cytoplasmic positivity in exocrine glandular cells at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with EIF4ENIF1 polyclonal antibody (Cat# PAB29326) under 1-4 ug/mL working concentration shows positivity in vesicles.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human EIF4ENIF1.
Immunogen
Recombinant protein corresponding to amino acids of human EIF4ENIF1.
Sequence
SPLEKDSDGLRLLGGRRIGSGRIISARTFEKDHRLSDKDLRDLRDRDRERDFKDKRFRREFGDSKRVFGERRRNDSYTEEEPEWFSAGPTSQSETIELTGFDDKILEEDHKGRKRTRRRTASVKEGIVECNGGVAEEDEVE
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Human cell line RT-4; Lane 2: Human cell line U-251MG sp; Lane 3: Human plasma (IgG/HSA depleted) with EIF4ENIF1 polyclonal antibody (Cat# PAB29326) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human pancreas with EIF4ENIF1 polyclonal antibody (Cat# PAB29326) shows strong cytoplasmic positivity in exocrine glandular cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with EIF4ENIF1 polyclonal antibody (Cat# PAB29326) under 1-4 ug/mL working concentration shows positivity in vesicles. -
Gene Info — EIF4ENIF1
Entrez GeneID
56478Protein Accession#
B1AKL4Gene Name
EIF4ENIF1
Gene Alias
4E-T, Clast4, FLJ21601, FLJ26551
Gene Description
eukaryotic translation initiation factor 4E nuclear import factor 1
Omim ID
607445Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
2610509L04Rik|OTTHUMP00000063276|eIF4E-transporter
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