EIF4ENIF1 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of EIF4ENIF1.
Immunogen
A synthetic peptide corresponding to human EIF4ENIF1.
Sequence
C-AKVISVDELEYRQ
Host
Goat
Theoretical MW (kDa)
108, 88.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (0.25-0.5 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
EIF4ENIF1 polyclonal antibody (Cat # PAB6555) staining (0.25 ug/mL) of 293 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
EIF4ENIF1 polyclonal antibody (Cat # PAB6555, 5 ug/mL) staining of paraffin embedded human pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EIF4ENIF1
Entrez GeneID
56478Protein Accession#
NP_062817.1Gene Name
EIF4ENIF1
Gene Alias
4E-T, Clast4, FLJ21601, FLJ26551
Gene Description
eukaryotic translation initiation factor 4E nuclear import factor 1
Omim ID
607445Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
2610509L04Rik|OTTHUMP00000063276|eIF4E-transporter
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Interactome
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Publication Reference
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A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E.
Dostie J, Ferraiuolo M, Pause A, Adam SA, Sonenberg N.
The EMBO Journal 2000 Jun; 19(12):3142.
Application:IF, IP, WB-Ce, Human, HeLa cells.
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A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E.
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