MDM2 (phospho S186/S188) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human MDM2.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S186/S188 of human MDM2.
Host
Rabbit
Theoretical MW (kDa)
55
Reactivity
Human, Mouse, Rat
Specificity
MDM2 (phospho S186/S188) polyclonal antibody detects endogenous levels of human MDM2 only when phosphorylated at serine 186 and serine 188.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100~1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells with MDM2 (phospho S186/S188) polyclonal antibody (Cat# PAB29284) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution. -
Gene Info — MDM2
Entrez GeneID
4193Protein Accession#
Q00987Gene Name
MDM2
Gene Alias
HDMX, MGC71221, hdm2
Gene Description
Mdm2 p53 binding protein homolog (mouse)
Omim ID
164785Gene Ontology
HyperlinkGene Summary
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq
Other Designations
Mdm2, transformed 3T3 cell double minute 2, p53 binding protein|double minute 2, human homolog of; p53-binding protein|mouse double minute 2 homolog|p53-binding protein MDM2|ubiquitin-protein ligase E3 Mdm2
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