MDM2 recombinant monoclonal antibody, clone 14F8
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More Files
- More Functions
Immunohistochemistry
Immunohistochemistry image of MDM2 recombinant monoclonal antibody, clone 14F8 diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of HepG2 Cells with MDM2 recombinant monoclonal antibody, clone 14F8 at 1:10, counter-stained with DAPI.
Flow Cytometry
Overlay Peak curve showing Hela cells stained with MDM2 recombinant monoclonal antibody, clone 14F8 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MDM2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human MDM2.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Immunohistochemistry(1:50-1:200)
Immunofluorescence(1:20-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunohistochemistry image of MDM2 recombinant monoclonal antibody, clone 14F8 diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of HepG2 Cells with MDM2 recombinant monoclonal antibody, clone 14F8 at 1:10, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay Peak curve showing Hela cells stained with MDM2 recombinant monoclonal antibody, clone 14F8 (red line) at 1:50. -
Gene Info — MDM2
Entrez GeneID
4193Protein Accession#
Q00987Gene Name
MDM2
Gene Alias
HDMX, MGC71221, hdm2
Gene Description
Mdm2 p53 binding protein homolog (mouse)
Omim ID
164785Gene Ontology
HyperlinkGene Summary
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq
Other Designations
Mdm2, transformed 3T3 cell double minute 2, p53 binding protein|double minute 2, human homolog of; p53-binding protein|mouse double minute 2 homolog|p53-binding protein MDM2|ubiquitin-protein ligase E3 Mdm2
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Interactome
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Pathway
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Disease
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