ITGB1 (phospho T789) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of Lane 1: Ca2+ treated HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with ITGB1 (phospho T789) polyclonal antibody (Cat# PAB29262) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with ITGB1 (phospho T789) polyclonal antibody (Cat# PAB29262) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human ITGB1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T789 of human ITGB1.
Host
Rabbit
Theoretical MW (kDa)
90
Reactivity
Human, Mouse, Rat
Specificity
ITGB1 (phospho T789) polyclonal antibody detects endogenous levels of human ITGB1 only when phosphorylated at threonine 789.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Ca2+ treated HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with ITGB1 (phospho T789) polyclonal antibody (Cat# PAB29262) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with ITGB1 (phospho T789) polyclonal antibody (Cat# PAB29262) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — ITGB1
Entrez GeneID
3688Protein Accession#
P05556Gene Name
ITGB1
Gene Alias
CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB
Gene Description
integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Omim ID
135630Gene Ontology
HyperlinkGene Summary
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000019420|OTTHUMP00000046253|OTTHUMP00000063731|OTTHUMP00000063732|OTTHUMP00000063733|fibronectin receptor beta subunit|integrin VLA-4 beta subunit|integrin beta 1
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