ITGB1 recombinant monoclonal antibody, clone 32A10
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: SH-SY5Y whole cell lysate and Lane 4: U251 whole cell lysate with ITGB1 recombinant monoclonal antibody, clone 32A10 (Cat # RAB04368).
Immunohistochemistry
Immunohistochemical staining of human liver cancer with ITGB1 recombinant monoclonal antibody, clone 32A10 (Cat # RAB04368) (diluated at 1:185).
Immunofluorescence
Immunofluorescent staining of Hela cells with ITGB1 recombinant monoclonal antibody, clone 32A10 (Cat # RAB04368) (diluated at 1:61). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with ITGB1 recombinant monoclonal antibody, clone 32A10 (Cat # RAB04368) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ITGB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ITGB1.
Theoretical MW (kDa)
Calculated MW: 89, 8
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: SH-SY5Y whole cell lysate and Lane 4: U251 whole cell lysate with ITGB1 recombinant monoclonal antibody, clone 32A10 (Cat # RAB04368).Immunohistochemistry
Immunohistochemical staining of human liver cancer with ITGB1 recombinant monoclonal antibody, clone 32A10 (Cat # RAB04368) (diluated at 1:185).Immunofluorescence
Immunofluorescent staining of Hela cells with ITGB1 recombinant monoclonal antibody, clone 32A10 (Cat # RAB04368) (diluated at 1:61). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with ITGB1 recombinant monoclonal antibody, clone 32A10 (Cat # RAB04368) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — ITGB1
Entrez GeneID
3688Protein Accession#
P05556Gene Name
ITGB1
Gene Alias
CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB
Gene Description
integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Omim ID
135630Gene Ontology
HyperlinkGene Summary
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000019420|OTTHUMP00000046253|OTTHUMP00000063731|OTTHUMP00000063732|OTTHUMP00000063733|fibronectin receptor beta subunit|integrin VLA-4 beta subunit|integrin beta 1
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Interactome
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Pathway
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Disease
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