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BCAR1 (phospho Y410) polyclonal antibody

  • Catalog # : PAB29258
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human BCAR1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y410 of human BCAR1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 130
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • BCAR1 (phospho Y410) polyclonal antibody detects endogenous levels of human BCAR1 only when phosphorylated at tyrosine 410.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunohistochemistry (1:50~1:100)
    Western Blot (1:500~1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: antigen-specific peptide treated K562 cells, Lane 2: K562 cells, Lane 3: 3T3 cells with BCAR1 (phospho Y410) polyclonal antibody (Cat# PAB29258) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with BCAR1 (phospho Y410) polyclonal antibody (Cat# PAB29258) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 9564
  • Gene Name:
  • BCAR1
  • Gene Alias:
  • CAS,CAS1,CASS1,CRKAS,P130Cas
  • Gene Description:
  • breast cancer anti-estrogen resistance 1
  • Gene Summary:
  • BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM
  • Other Designations:
  • Cas scaffolding protein family member 1,Crk-associated substrate p130Cas
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