Western blot analysis of Lane 1: antigen-specific peptide treated Hela cells, Lane 2: HeLa cells, Lane 3: A549 cells, Lane 4: HepG2 cells with TOB1 (phospho S164) polyclonal antibody (Cat # PAB29253) at 1:500-1000 dilution.
Immunohistochemical staining of human brain tissue by TOB1 (phospho S164) polyclonal antibody (Cat # PAB29253) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
This gene encodes a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells. [provided by RefSeq