TOB1 (phospho S164) polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated Hela cells, Lane 2: HeLa cells, Lane 3: A549 cells, Lane 4: HepG2 cells with TOB1 (phospho S164) polyclonal antibody (Cat # PAB29253) at 1:500-1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by TOB1 (phospho S164) polyclonal antibody (Cat # PAB29253) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human TOB1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S164 of human TOB1.
Host
Rabbit
Theoretical MW (kDa)
40
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of TOB1 only when phosphorylated at serine 164.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated Hela cells, Lane 2: HeLa cells, Lane 3: A549 cells, Lane 4: HepG2 cells with TOB1 (phospho S164) polyclonal antibody (Cat # PAB29253) at 1:500-1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by TOB1 (phospho S164) polyclonal antibody (Cat # PAB29253) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — TOB1
Entrez GeneID
10140Protein Accession#
P50616Gene Name
TOB1
Gene Alias
APRO6, MGC104792, MGC34446, PIG49, TOB, TROB, TROB1
Gene Description
transducer of ERBB2, 1
Omim ID
605523Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells. [provided by RefSeq
Other Designations
transducer of erbB-2
-
Interactome
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com