Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with PSMD5 polyclonal antibody (Cat # PAB28719) at 1:100-1:500 dilution.
Immunohistochemical staining of human prostate with PSMD5 polyclonal antibody (Cat # PAB28719) shows strong strong nuclear positivity in glandular cells at 1:50-1:200 dilution.
Immunofluorescent staining of human cell line A-431 with PSMD5 polyclonal antibody (Cat # PAB28719) at 1-4 ug/mL dilution shows positivity in nucleus and cytoplasm.
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator base. [provided by RefSeq