PHKG1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PHKG1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human PHKG1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PHKG1 polyclonal antibody (Cat # PAB2794) in A-375 cell lysate. PHKG1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PHKG1 polyclonal antibody (Cat # PAB2794) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — PHKG1
Entrez GeneID
5260Protein Accession#
Q16816Gene Name
PHKG1
Gene Alias
PHKG
Gene Description
phosphorylase kinase, gamma 1 (muscle)
Omim ID
172470Gene Ontology
HyperlinkGene Summary
This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. [provided by RefSeq
Other Designations
phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform|phosphorylase kinase gamma subunit 1
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Interactome
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Pathway
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Publication Reference
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Localisation of the gene encoding the catalytic gamma subunit of phosphorylase kinase to human chromosome bands 7p12-q21.
Jones TA, da Cruz e Silva EF, Spurr NK, Sheer D, Cohen PT.
Biochimica et Biophysica Acta 1990 Jan; 1048(1):24.
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Localisation of the gene encoding the catalytic gamma subunit of phosphorylase kinase to human chromosome bands 7p12-q21.
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