PRKCQ polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of exreacts from HT-29 and K-562 cells untreated or treated with anisomycin (1mM, 30 min) using PRKCQ polyclonal antibody (Cat # PAB26739).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PRKCQ polyclonal antibody (Cat # PAB26739).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKCQ.
Immunogen
A synthetic peptide corresponding to residues surrounding S695 of human PRKCQ.
Sequence
N-F-Sp-F-M
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity chromatography
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of exreacts from HT-29 and K-562 cells untreated or treated with anisomycin (1mM, 30 min) using PRKCQ polyclonal antibody (Cat # PAB26739).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PRKCQ polyclonal antibody (Cat # PAB26739). -
Gene Info — PRKCQ
Entrez GeneID
5588Protein Accession#
Q04759Gene Name
PRKCQ
Gene Alias
MGC126514, MGC141919, PRKCT, nPKC-theta
Gene Description
protein kinase C, theta
Omim ID
600448Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq
Other Designations
OTTHUMP00000019053|OTTHUMP00000043364|OTTHUMP00000043365
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